Considering the importance of nanotechnology, especially nanofibers and nanocomposites ; as its most eligible products and the vast applications of polyvinyl alcohol (PVA) and nanoclay ( modified organo-clay), the main objective of this work was study on effect of polymer blends (PVA as matrix) and nano-filler ( organo-clay as disperse phase) concentrations and electrospinning parameters , on morphology of resulted nanocomposite nanofibers. It is noteworthy that bead less nanofibers with even diameter distributions can make the final products application more valuable. In order to investigate the microstructure and morphology and the miscibility and chemical bonding of polyvinyl alcohol / organo-clay nanocomposite nanofibers , the scanning election microscope (SEM), and the Fourier transform infrared spectrometer (FTIR) were used , respectively. Also, the thermal gravimetric analysis (TGA) was employed to investigate thermal behavior of nanocomposite nanofiber samples. The X-Ray Diffraction (XRD) data demonstrated the exfoliation of organo-clay layers in poly vinylalcohol matrix and nanofibers nanocomposite were electrospun with diameter about 300 nm.